Technology for single-cell analysis


The center develops single-cell proteomics technology that is quantitatively accurate, scalable, robust, and easy to adopt. It includes:


Shotgun single-cell proteomics

SCoPE-MS and its second version SCoPE2 can analyze thousands of proteins selected in order of their abundance in the samples. SCoPE2 uses multiplexed experimental designs in which proteins from single cells and from a small group of cells (called carrier proteins) are barcoded with isobaric mass tags and then combined. This design, utilizes the isobaric carrier to reduce the loss of proteins from single cells adhering to equipment surfaces while simultaneously enhancing peptide identification.

Single Cell ProtEomics by Mass-Spectrometry

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Prioritized single-cell proteomics

pSCoPE can analyze thousands of proteins selected in order of their priority for the biological question and project of interest.

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Parallel analysis of both single cells and peptides

plexDIA parallelizes the analysis of both single cells and peptides. It quantifies about a thousand proteins per single cell while achieving 98% data completeness within a set. It does not use a carrier sample.

Single Cell ProtEomics by plexDIA

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Sample preparation for single-cell proteomics

We have developed and use methods that use solely MS-compatible reagents and allow parallel preparation of hundreds of single cells in small volumes. These include minimal sample preparation and droplet sample preparation.

Sample preparation for Single Cell Proteomics

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About the single-cell proteomics center

The single-cell proteomics center is located at Northeastern University in Boston, MA, USA. Questions about the center should be addressed to Prof. Nikolai Slavov.